anti alpha smooth muscle actin α sma mouse monoclonal antibody  (Boster Bio)

Journal: bioRxiv

Article Title: Identification of Sensory Fiber Types in Mouse Temporomandibular Joint Tissues

doi: 10.1101/2025.05.15.654333

Figure Lengend Snippet: ( A ) The left column shows pgp9.5-positive fibers (all sensory fibers), the middle column shows PV-positive fibers in PV/tdTom mouse and the right column shows co-expressions of pgp9.5 and PV-positive fibers in the mouse joint capsule, anterior disc and retrodiscal tissues. Pictures from the joint capsule, anterior disc and retrodiscal tissues as well as antibodies used and corresponding colors are indicated. White arrows show pgp9.5 + /PV - nerves. ( B ) Percentages of pgp9.5 + and tyrosine hydroxylase (TH)-positive nerves in a vicinity of the blood vessels (α-SMA + ) in the joint capsule, anterior disc and retrodiscal tissues. Statistics is 1-way ANOVA Bonferroni’s pot-hoc test (n=4).

Article Snippet: The following well-characterized primary antibodies were used on mouse tissue sections: anti-neurofilament heavy chain (NFH) chicken polyclonal antibodies (BioLegend, catalog #PCK-592P, 1:300) ; anti-pgp9.5 (Millipore-Sigma, catalog #AB1761-I, 1:400) ; anti-CGRP rabbit polyclonal (Sigma, C8198, 1:300) – ; anti-tyrosine hydroxylase (TH) rabbit polyclonal (Pel-Freez; Rogers, AR; P40101; 1:400) , ; and anti-smooth muscle actin (α-SMA) Cy3-conjugated mouse monoclonal antibody (Sigma, C6198, 1:200) , .

Techniques:

Journal: bioRxiv

Article Title: Identification of Sensory Fiber Types in Mouse Temporomandibular Joint Tissues

doi: 10.1101/2025.05.15.654333

Figure Lengend Snippet: The left column shows pgp9.5-positive fibers (all sensory fibers), the middle column shows α-SMA-positive (blood vessel) cells and the right column shows relative locations of pgp9.5 and α-SMA-positive cells in the mouse joint capsule, articular disc, anterior disc and retrodiscal tissues. Pictures from the joint capsule, articular disc, anterior disc and retrodiscal tissues as well as antibodies used and corresponding colors are indicated. Yellow arrows show pgp9.5 + fibers in vicinity of α-SMA + blood vessels, and white arrows show pgp9.5 + fibers distanced from α-SMA + blood vessels.

Article Snippet: The following well-characterized primary antibodies were used on mouse tissue sections: anti-neurofilament heavy chain (NFH) chicken polyclonal antibodies (BioLegend, catalog #PCK-592P, 1:300) ; anti-pgp9.5 (Millipore-Sigma, catalog #AB1761-I, 1:400) ; anti-CGRP rabbit polyclonal (Sigma, C8198, 1:300) – ; anti-tyrosine hydroxylase (TH) rabbit polyclonal (Pel-Freez; Rogers, AR; P40101; 1:400) , ; and anti-smooth muscle actin (α-SMA) Cy3-conjugated mouse monoclonal antibody (Sigma, C6198, 1:200) , .

Techniques:

Journal: bioRxiv

Article Title: Identification of Sensory Fiber Types in Mouse Temporomandibular Joint Tissues

doi: 10.1101/2025.05.15.654333

Figure Lengend Snippet: The left column shows TH-positive fibers (sympathetic nerves), the middle column shows α-SMA-positive (blood vessel) cells and the right column shows relative locations of TH and α-SMA-positive cells in the mouse joint capsule, articular disc, anterior disc and retrodiscal tissues. Pictures from the joint capsule, articular disc, anterior disc and retrodiscal tissues as well as antibodies used and corresponding colors are indicated. Yellow arrows show TH + fibers in vicinity of α-SMA + blood vessels, and white arrows show TH + fibers distanced from α-SMA + blood vessels.

Article Snippet: The following well-characterized primary antibodies were used on mouse tissue sections: anti-neurofilament heavy chain (NFH) chicken polyclonal antibodies (BioLegend, catalog #PCK-592P, 1:300) ; anti-pgp9.5 (Millipore-Sigma, catalog #AB1761-I, 1:400) ; anti-CGRP rabbit polyclonal (Sigma, C8198, 1:300) – ; anti-tyrosine hydroxylase (TH) rabbit polyclonal (Pel-Freez; Rogers, AR; P40101; 1:400) , ; and anti-smooth muscle actin (α-SMA) Cy3-conjugated mouse monoclonal antibody (Sigma, C6198, 1:200) , .

Techniques:

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Construction of a Biomimetic Tubular Scaffold Inspired by Sea Sponge Structure: Sponge-Like Framework and Cell Guidance.

doi: 10.1002/advs.202416627

Figure Lengend Snippet: Figure 5. Cultivation of vascular cells on SO-(oxBC/BC)@PDA hollow fibers. a) Cell viability of ECs and SMCs co-cultured with SO-oxBC/BC and SO- (oxBC/BC)@PDA fibers. b) Fluorescence images of ECs on SO-(oxBC/BC)@PDA fibers: b1–b3) CD31 and b4–b6) F-actin. c) Images of SMCs on SO- (oxBC/BC)@PDA fibers: c1) SEM images showing spiral ridge structures and c2–c4) fluorescence images of F-actin expression in SMCs. d) Schematic diagram of SMCs orientation on SO-(oxBC/BC)@PDA fibers. e) Comparison of the orientation direction of oxBC nanofibrils and SMCs on the fiber surface. f) Images of SMCs on AO-(oxBC/BC)@PDA fibers: f1) SEM images without spiral ridge structures and f2–f4) fluorescence images of F-actin

Article Snippet: CD31 mouse monoclonal antibody, smooth muscle actin rabbit polyclonal, CyTM2conjugated AffiniPure Donkey Anti-Mouse lgG(H+L), and CoraLite594conjugated Goat Anti-Rabbit lgG(H+L) were purchased from Proteintech Co., Ltd., America.

Techniques: Cell Culture, Fluorescence, Expressing, Comparison